Fig. 7. Twist1 overexpression reverses the inhibitory effects of shNF-κB combined with ChR on EMT and CSLC features in HeLa cells induced by TNF-α and TGF-β co-treatment. HeLa cells co-transduced with adenoviruses carrying the Twist1 and shNF-κBp65 genes were co-administered TNF-α and TGF-β, with or without ChR(10.0μM). Then, cell morphology (A, scale bar, 50 μm), E-cadherin and N-cadherin protein amounts (B), NF-κBp65 and Twist1 protein levels (C), cell migration (D) and self-renewal (E, scale bar, 200 μm) abilities, and the protein expression levels of Bmi1, Sox2 and Oct4(F), as well as CD133, ALDH1, and CD44 (G) were determined. β-actinserved as an internal reference for immunoblots. cDNA, cells transduced with adenoviruses expressing Twist1; cDNA+shRNA, cells co-transduced with adenoviruses harboring the Twist1 and shNF-κBp65 genes.^*p<0.05 vs shNF-κBp65. ^#p<0.05 vs shNF-κBp65 with ChR(10.0μM) treatment.